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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in endorsing mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two extra splice variants were claimed but specific nucleotide sequence info is just not but available.
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Blocking peptides are peptides that bind precisely into the goal antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This guideline outlines even further attainable optimization of mobile and immunoassay parameters to be certain the very best outcomes are...
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This information outlines further more attainable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
Antibodies sure to the blocking peptide no more bind for the epitope on the concentrate on protein. By comparing the staining within the blocked antibody compared to the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs towards the very conserved cyclin loved ones, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation designs which contribute for the temporal coordination of each mitotic function. Cyclin E kinds a complex with and functions being a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S changeover. This protein accumulates in the G1-S section boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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